Comparison of Conventional, Nested, and Real-Time Quantitative PCR for Diagnosis of Scrub Typhus
نویسندگان
چکیده
منابع مشابه
Comparison of conventional, nested, and real-time quantitative PCR for diagnosis of scrub typhus.
Orientia tsutsugamushi is the causative agent of scrub typhus. For the diagnosis of scrub typhus, we investigated the performances of conventional PCR (C-PCR), nested PCR (N-PCR), and real-time quantitative PCR (Q-PCR) targeting the O. tsutsugamushi-specific 47-kDa gene. To compare the detection sensitivities of the three techniques, we used two template systems that used plasmid DNA (plasmid d...
متن کاملUsefulness of eschar PCR for diagnosis of scrub typhus.
We report here on the case of a child who was infected with scrub typhus, and we made the diagnosis according to the serology and by performing PCRs on the child's eschar. The patient was treated with azithromycin, and he did not experience any complications. Performing nested PCR on the eschar might be both a rapid diagnostic test for scrub typhus in the early acute stage and a differential te...
متن کاملDiagnosis of Scrub Typhus
Scrub typhus is transmitted by trombiculid mites and is endemic to East and Southeast Asia and Northern Australia. The clinical syndrome classically consists of a fever, rash, and eschar, but scrub typhus also commonly presents as an undifferentiated fever that requires laboratory confirmation of the diagnosis, usually by indirect fluorescent antibody (IFA) assay. We discuss the limitations of ...
متن کاملDevelopment of Conventional and Real-Time Quantitative PCR Assays for Diagnosis and Monitoring of Scabies.
Scabies remains the most prevalent, endemic, and neglected ectoparasitic infestation globally and can cause institutional outbreaks. The sensitivity of routine microscopy for demonstration of Sarcoptes scabiei mites or eggs in skin scrapings is only about 50%. Except for three studies using conventional or two-tube nested PCR on a small number of cases, no systematic study has been performed to...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2011
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.01216-09